The BCA Protein Assay Kit provides a simple, rapid, detergent tolerant (up to 5%) procedure for determining the concentration of proteins in solution. The method utilizes a copper (Cu2+) salt which can be reduced to the cuprous state by protein(s). The generated Cu+ ion forms an intensely colored complex with the bicinchoninic acid reagent with a very strong absorbance band centered at 562nm. The intensity of the blue complex is proportional to the amount of protein in the sample. The BCA Protein Assay is suitable for measuring protein concentration in the range of 0.5-30ug protein (0.01-0.6mg/ml).Kit Components:BCA Reagent 1x100mlCopper Reagent BSA 1x2ml*Standard (10mg/ml) 1x1mlStorage and Stability:Store *Standard at -20°C. Store other components at RT. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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