FLAG-tag is a tag that can be added to a protein sequence motif DYKXXD. It can be used for affinity chromatography, to separate recombinant protein from wt proteins. It can also be used to isolate protein complexes with multiple subunits.
应用类型
Dot blot (Dot), ELISA (ELISA), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western blot (WB)
免疫原
KLH-conjugated DYKDDDDK (FLAG) synthetic peptide
来源宿主
小鼠
反应性
N-terminal, C-terminal or internal DYKDDDDK-tagged fusion proteins