Armenian hamster monoclonal [22F6] antibody to ZNFN1A2/HELIOS.
应用类型
Flow Cytometry: 1-4 ug/ml, Recommended protocol: 1) Perform staining of cell surface markers (CD25, CD4 etc.) for 20 min. at room temperature in the dark; 100 ul of peripheral blood. 2) Add 3 ml of PBS with 1% BSA, centrifugate at 300g and discard the supernatant. Further steps perform on ice and with ice-cold reagents. 3) Resuspend the cells in 5 ml of cold fixation solution (Miltenyi Biotec) and incubate for 30 min. on ice. 4) Centrifugate for 5 min. at 1000 g, 4
免疫原
Synthetic peptide corresponding to amino acids 51-107 of Helios.
