ELISA: This antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human CD14 in combination with antibody MEM-18 (cat. no. 1B-212-C100), Flow Cytometry: 1 ug/ml;IHC-P: 2-10 ug/ml, Positive Control: PML brain sections; positive staining on human PML brain sections was mainly observed on monocytes in the lumenal side of brain blood vessels, and on some perivascular cells adjacent to medium-sized vessels. Heat retrieval of antigen is recommeded.