Flow Cytometry: 1 ug/ml; Positive Control: peripheral blood lymphocytes, IP: Positive Control: granulocytes, CD11b/CD18 transfectants, Immunoprecipitation with the antibody MEM-174 was successfuly performed using immuno-affinity sorbents (minicolumns). The final immunosorbent should contain 1-5 mg of antibody/1 ml of sorbent gel (e.g. agarose beads). It is recommended to stimulate Positive Control material by LPS 1-2 days before to achieve better expression of target antigen.
