- 中文名称
JOE phosphoramidite, 6-isomer
- 英文名字
- JOE phosphoramidite, 6-isomer
- 供应商
- antibodies
- 产品货号
- ANT-A270233-5
- 产品报价
- ￥询价/5g
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系。本公司所有产品仅供科研使用，不用于临床诊断。
- 产品新闻
- 背景资料
- 该JOE phosphoramidite, 6-isomer的背景资料请参考antibodies.com的说明书
- 产品描述
- JOE phosphoramidite for oligonucleotide synthesis, pure 6-isomer (6-JOE). Fluorescent dye JOE is a fluorescein derivative containing two chlorine atoms and two methoxy groups. Its absorption and emission maxima are at 503 nm and 525 nm, respectively. By its spectral characteristics JOE is found in between FAM and TAMRA/ROX; therefore, this fluorophor is commonly used for multiplex detection, including that during DNA sequencing. Coupling time of 6 minutes. Deprotection can be perfromed under standard conditions using ammonium hydroxide; deprotection time depends on oligonucleotide composition and nucleobase protecting groups (deprotection for 17 h at 55 °C removes all protecting groups from standard nucleobases). AMA (solution of 30% ammonium hydroxide/40% aqueous methylamine 1:1 v/v) can be used with ~5% of non-fluorescent side product forming. To avoid formation of the side product, start deprotection with ammonium hydroxide (30 min at room temperature), then add an equal volume of 40% aqueous methylamine and continue deprotection as required with AMA (e.g. 10 min at 65 °C).
- 产品形式
- 保存建议
- 该JOE phosphoramidite, 6-isomer的保存建议请参考antibodies.com的说明书
- 其他
- antibodies.com 以具有成本效益的价格向生命科学研究人员提供高质量的生物试剂。公司总部位于剑桥（英国），并在斯德哥尔摩（瑞典）和圣路易斯（美国密苏里州）设有办事处，可提供包括一抗、二抗、蛋白质和肽、免疫分析和试剂盒以及其他相关产品。
- 注意
-
该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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