IGFBP-3，完整 ELISA-IGFBP-3, Intact ELISA代理/参数/厂家-ELISA试剂盒-艾美捷科技有限公司

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中文名称: IGFBP-3，完整 ELISA

英文名字: IGFBP-3, Intact ELISA

供应商: Ansh Labs

产品货号: AL-149

产品报价: ￥询价/96wellmicrotiter

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背景资料: Insulin-like growth factor binding protein-3 (IGFBP-3) is a 264-amino acid peptide (MW 29 kD) produced by the liver as well as other tissues. It is the most abundant of a group of IGFBPs that transport, and control bioavailability and half-life of insulin-like growth factors (IGF), in particular IGF-I, the major mediator of the anabolic- and growth-promoting effects of growth hormone (GH).1 The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II, thus prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Most of the IGFBP-3 in plasma is present as the high molecular weight ternary complex, however, small amounts of free IGFBP-3 are also found.2,3
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nIGFBP-3 also exhibits intrinsic growth-regulating effects that are not yet fully understood, but have evoked interest with regards to a possible role of IGFBP-3 as a prognostic tumor marker. Low IGFBP-3 and IGF-I levels are observed in GH deficiency or GH resistance,4 also epidemiologic studies suggest that low IGFBP-3 is associated with greater risk of aggressive, metastatic prostate cancers.5 The elevated serum IGFBP-3 and IGF-I levels indicate a sustained overproduction of GH, or excessive rhGH therapy. Both conditions are associated with generalized organomegaly,6 hypertension,7 diabetes,8 cardiomyopathy,9 osteoarthritis,10 and diminished longevity.
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nThe Ansh Labs Intact IGFBP-3 Assay uses an acidification and neutralization method to dissociate Intact IGFBP-3 from all the binding subunits. Intact IGFBP-3 levels are quantified in the extracted samples using a highly sensitive and specific Intact IGFBP-3 ELISA. Total IGFBP-3 levels can be measured using Total IGFBP-3 ELISA (AL-120).
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nThe concentration of bioactive IGF-I, total IGFBP-3 and intact IGFBP-3 in biological fluid can be measured accurately using immunoassay methods (Bioactive IGF-1 ELISA; AL-122, IGF-I inhibition mAb capture and IGF-1 C-terminal mAb) detection; Total IGFBP-3 ELISA; AL-120, C-terminal IGFBP-3 capture and detection mAb; and Intact IGFBP-3 ELISA; AL-149, C-terminal mAb capture and N-terminal mAb detection, respectively). The ratio of total to Intact IGFBP-3 concentration measured in individual subject over time will help normalizes the IGFBP-3 variability between subjects. The immunoassay methods designed for the measurement of bioactive IGF-I, total and Intact IGFBP-3 in patient samples could be of practical value for the diagnosis or prediction of various pathologies including growth abnormalities and cancer.
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nReferences:
n1. Hwa V, Oh Y, Rosenfeld RG. The insulin-like growth factor-binding protein (IGFBP) superfamily, Endocr Rev. 1999 Dec;20 (6):761-87.
n2. Baxter RC, Martin J : Radioimmunassay of growth hormone-dependent insulin-like growth factor binding protein in human plasma. J Clin Invest 1986, 78:1504-1512
n3. Blum WF, Ranke MB, Kietzmann K, Gauggel E, Zeissel HJ, Bierich JR. A specific radioimmunoassay for the growth hormone (GH)-dependent somatomedin-binding protein: its use for diagnosis of GH deficiency. J Clin Endocrinol Metab, 1990, 70:1292-1298
n4. Wetterau L, Cohen P: Role of insulin-like growth factor monitoring in optimizing growth hormone therapy. J Ped Endocrinol Metab 2000;13:1371-1376
n5. Cancer Res August 1, 2011 71;5154 IGFBP-3 Is a Metastasis Suppression Gene in Prostate Cancer
n6. Parama C, Fluiters E, de la Fuente J, et al: Monitoring of treatment success in patients with acromegaly: the value of serum insulin-like growth factor binding protein-3 and serum leptin measurements in comparison to plasma insulin-like growth factor 1 determination. Metabolism 2001; 50: 1117-1121.
n7. Watanabe T1, Itokawa M, Nakagawa Y, Iguchi T, Katagiri T. Increased levels of insulin-like growth factor binding protein-3 in hypertensive patients with carotid atherosclerosis. Am. J. Hypertens. 2003 Sep; 16 (9 Pt 1):754-60.
n8. Kim MS, Lee DY. Serum insulin-like growth factor-binding protein-3 level correlated with glycemic control and lipid profiles in children and adolescents with type 1 diabetes. J Pediatr Endocrinol Metab. 2014 Sep 20;27(9-10):857-61. doi: 10.1515/jpem-2013-0358.
n9. Granata R, Broglio F, Migliorino D, Cutrupi S, Baldanzi G, Sireno M, Fubini A, Grazian A, Ghigo E, Pucci A. Neonatal and adult human heart tissues from normal subjects and patients with ischemic, dilated or hypertrophic cardiomyopathy express insulin-like growth factor binding protein-3 (IGFBP-3). J Endocrinol Invest. 2000 Dec;23(11):724-6.
n10. Lee HS, Woo SJ, Koh HW, Ka SO, Zhou L, Jang KY, Lim HS, Kim HO, Lee SI, Park BH. Regulation of apoptosis and inflammatory responses by insulin-like growth factor binding protein 3 in fibroblast-like synoviocytes and experimental animal models of rheumatoid arthritis. Arthritis Rheumatol. 2014 Apr;66(4):863-73. doi: 10.1002/art.38303.
n11. HHS Publication, 5th ed., 2007. Biosafety in Microbiological and Biomedical Laboratories. Available http://www.cdc.gov/biosafety/publications/bmbl5/BMBL5
n12. DHHS (NIOSH) Publication No. 78–127, August 1976. Current Intelligence Bulletin 13 - Explosive Azide Hazard. Available http:// www.cdc.gov/niosh.
n13. Kricka L. Interferences in immunoassays – still a threat. Clin Chem 2000; 46: 1037–1038.

产品描述: The Intact IGFBP-3 enzyme linked immunosorbent assay (ELISA) kit provides materials for the quantitative measurement of intact IGFBP-3 in human serum and other biological fluids.

产品特点

保存建议: Store at 2 to 8°C until expiration date.

其他: Ansh labs是开发和生产免疫检测试剂盒和定制生物技术检测产领创者，总部位于美国休斯敦，公司产品遍及全球80多个国家。公司于2011年成立，核心团队来自于Diagnostic system laboratory，专注于女性健康和激素检测领域近40年，拥有全球领先的核心技术，以及多年经验生物技术研发经验的科学家团队，产品覆盖生殖健康，高危妊娠，肿瘤，糖尿病等多个疾病领域，围绕最新的免疫分析技术，多种蛋白和单克隆抗体，如AMH，BMP-15, Follistatin, GDF-9, IGF-I, IGF-II, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-5, Inhibin Alpha, Inhibin ?A, Inhibin ?B, MBP, PAPP-A, PAPP-A2 and Vitamin D.等几十余种试剂产品，也同时涵盖小鼠，大鼠，狗，羊，牛等灵长类多个物种的独特的物种特异性的试剂。 Ansh labs 坐落于美国德克萨斯州，是目前世界上唯一能提供TGF-β超家族所有成员的检测公司，也是最早专注激素类产品研究和新型免疫检测技术的先驱者。优势产品涉及TGF-β super family、AMH/抑制素B(human/animal)、full panel IGFs、以及最完整的胰高血糖素系列。