Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. MRPL33(mitochondrial ribosomal protein L33) encodes a 39S subunit protein. Alternatively spliced transcript variants encoding different isoforms have been described.蛋白别名为:MRPL33; C2orf1; 39S ribosomal protein L33; mitochondrial; L33mt; MRP-L33;基因ID为:9553;蛋白质ID:O75394
应用类型
IHC-p,ELISA补充:最优的抗体稀释比例需要基于客户实验进行优化.建议的起始稀释比例如下: IHC-p: 1:100-1:300, ELISA: 1:10000. Not yet tested in other applications.
免疫原
合成多肽:the N-terminal region of human MRP-L33. at AA rangle: 20-100