Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. MRPL47(mitochondrial ribosomal protein L47) encodes a 39S subunit protein. MRPL47 is immediately adjacent to the gene for BAF complex 53 kDa subunit protein a (BAF53a), in a tail-to-tail orientation. Two transcript variants encoding different protein isoforms have been identified.蛋白别名为:MRPL47; NCM1; CGI-204; 39S ribosomal protein L47; mitochondrial; L47mt; MRP-L47; Nasopharyngeal carcinoma metastasis-related protein 1;基因ID为:57129;蛋白质ID:Q9HD33
应用类型
WB,ELISA补充:最优的抗体稀释比例需要基于客户实验进行优化.建议的起始稀释比例如下: WB: 1:500-1:2000, ELISA: 1:10000. Not yet tested in other applications.
免疫原
合成多肽:the C-terminal region of human MRP-L47. at AA rangle: 160-240