SPI1 encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. Spi-1 proto-oncogene can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this SPI1.蛋白别名为:SPI1; Transcription factor PU.1; 31 kDa-transforming protein;基因ID为:6688;蛋白质ID:P17947
应用类型
WB,IHC-p,IF,ELISA补充:最优的抗体稀释比例需要基于客户实验进行优化.建议的起始稀释比例如下: WB: 1:500-1:2000, IHC-p: 1:100-1:300, IF: 1:200-1:1000, ELISA: 1:40000. Not yet tested in other applications.
免疫原
合成多肽:the Internal region of human PU.1. at AA rangle: 150-230