CRK encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of CRK(CRK proto-oncogene, adaptor protein) has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described.蛋白别名为:CRK; Adapter molecule crk; Proto-oncogene c-Crk; p38;基因ID为:1398;蛋白质ID:P46108
应用类型
WB,IF,ELISA补充:最优的抗体稀释比例需要基于客户实验进行优化.建议的起始稀释比例如下: WB: 1:500-1:2000, IF: 1:200-1:1000, ELISA: 1:40000. Not yet tested in other applications.
免疫原
合成多肽:human Crk II around the phosphorylation site of Y221.