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中文名称
红色活细胞荧光探针CytoTell Red
英文名字
CytoTell Red
供应商
AAT Bioquest
产品货号
AAT-22255
产品报价
¥1474/500Tests
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产品新闻
背景资料
Flow cytometry combined with fluorescence staining is a powerful tool to analyze heterogeneous cell populations。 Among all the existing fluorescent dyes CFSE is the preferred cell proliferation indicator that is widely used for live cell analysis。 However, it is impossible to use CFSE and its fluorescein analogs for GFP-transfected cells or for the applications where a FITC-labeled antibody is used since CFSE and its fluorescein analogs have the excitation and emission spectra almost identical to GFP or FITC。 CytoTell dyes are well excited at major laser lines such as 405 nm, 488 nm or 633 nm with multicolor emissions。 CytoTell dyes have minimal cytotoxicity, and are used for the multicolor applications with either GFP cell lines or FITC-labeled antibodies since they have either excitation or emission spectra distinct from fluorescein。 CytoTell Red is a red fluorescent dye that stains cells evenly。 As cells divide, the dye is distributed equally between daughter cells that can be measured as successive halving of the fluorescence intensity of the dye。 Up to 8 generations may be visualized。 CytoTell Red can also be used for long term tracking of labeled cells。 Analysis using two-parameter plots may provide better resolution of each generation, especially between undivided cells and the first generation。 Cells labeled with Cell CytoTell Red may be fixed and permeabilized for analysis of intracellular targets using standard formaldehyde-containing fixatives and saponin-based permeabilization buffers。 CytoTell Red has a peak excitation of 630 nm and can be excited by the red (633 nm) laser line。 It has a peak emission of 660 nm and can be detected with a 660/20 band pass filter (equivalent to APC, Alexa Fluor 647, or Cy5), making it compatible with applications that utilize GFP or FITC antibodies for multicolor cell analysis。
产品描述
红色活细胞荧光探针CytoTell Red产品的光谱学相关参数为Ex(nm)=628/ Em(nm)643;其对应分子量大小为600。
产品形式
提供的红色活细胞荧光探针CytoTell Red产品为固体形式,我们建议将其溶解到DMSO溶液中使用或保存。
保存建议
对于红色活细胞荧光探针CytoTell Red产品,我们推荐使用常温进行运输。在您收到货物后,我们建议在低于-15℃温度下冷冻保存,要求储存环境干燥并避免光照。产品有效期为12个月。
其他
美国AAT Bioquest Inc。(前身是ABD Bioquest,Inc。)是一家为从事生命科学研究、诊断研发及药物开发的科学家研发、生产和销售生物分析研究试剂和试剂盒的公司。公司致力于光谱学检测领域,包括显色、荧光和生物发光技术。AAT Bioquest的产品帮助全世界的科学家和生物医药研究者更好的了解生物化学,免疫学,细胞生物学和分子生物学等领域。作为AAT Bioquest Inc。的中国区域代理,艾美捷科技为中国客户提供光谱学检测技术并应用于生化、生理代谢和细胞分析领域的产品,包括显色,荧光和发光技术等全系列解决方案。
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