Microspheres are routinely coated with ligands such as antibodies, oligonucleotides and peptides for use in diagnostics and bioseparations. Carboxyl- and amine-modified microspheres require the use of a chemical linker for activation and covalent immobilization of ligand. DEPC-Carbodiimide (EDAC) and Glutaraldehyde are two of the most common crosslinkers used in bead coating protocols. To purchase as a kit, see our coupling kits. EDAC is a zero-length crosslinker that is routinely used for the covalent binding of amine-containing ligands to carboxylated microspheres. Glutaraldehyde is a homobifunctional linker that is suitable for binding amine-containing ligands to amine-modified beads.