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细胞分析试剂盒
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中文名称

Antioxidant Quantification, Aqueous Kit, Total, BioAssay

英文名字
Antioxidant Quantification, Aqueous Kit, Total, BioAssay
供应商
USBiological
产品货号
A2298-46
产品报价
¥1/1Kit
产品说明书
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产品新闻
背景资料
USBiological品牌是全球有名的抗原抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理,是行业中少有的致力于服务客户,帮助客户,且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应,专业的产品和解决方案支持,稳健快捷的交货周期,优质放心的售后服务。我们致力于为您提供有价值的产品和服务,在意您的成功!
产品描述
This TAP assay allows the accurate measure a sample’s total antioxidant activity, which is the sum of the combined antioxidant activities of all of the samples constituents, including vitamins, proteins, glutathione, etc. This method can be used to quantify the antioxidant capacity for a wide range of biological fluids as well as foods and nutritional supplements.Oxidative stress is the condition in which a lack of balance exists between the oxidant stimuli and the various antioxidants (1). Oxidative stress appears to be involved in the pathogenesis of several diseases, including artherosclerosis (2), chronic inflammatory diseases (3), and cancer (4). Therefore, the body possesses several antioxidant systems that are very important for the prevention of oxidative stress (5). These antioxidant systems include enzymatic (prevalently, if not exclusively, intracellular) and non-enzymatic (both intra- and extracellular). Lipid soluble antioxidants (most importantly vitamin E) and water-soluble antioxidants (uric acid, vitamin C, bilirubin, thiols and gluthathione) are involved in these processes. Given the multiplicity of antioxidant pathways, their centrality in the prevention of oxidant stress, and the influences of lifestyle and nutritional supplements on an individual’s antioxidant capacity, it is important to be able to quantitatively measure the total antioxidant capacity or antioxidant power within biological specimens (6-11).Principle of the Assay:The reduction potential of the sample or standard effectively converts Cu+2 to Cu+1, thus changing the ion’s absorption characteristics. This reduced form of copper will selectively form a stable 2:1 complex with the chromogenic reagent with an absorption maximum at ~450 nm (12-16). A known concentration of Trolox is used to create a calibration curve, with the data being expressed as uM Trolox equivalents or in uM copper reducing equivalents.Kit Components:A2298-46A: DILUTION BUFFER, 1x60ml A2298-46B: COPPER SOLUTION, 1x5ml A2298-46C: STOP SOLUTION, 1x5ml *A2298-46D: Trolox STANDARD, 1x1vialA22298-46E: Microtiter Plate, 1x 96wellStorage and Stability:Store *A2298-46D powder at 4°C, liquid at -70°C. Store other components at 4°C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
产品特点
针对Antioxidant Quantification, Aqueous Kit, Total, BioAssay该产品的特点优势,欢迎查阅官网提供的产品说明书。
保存建议
建议收到Antioxidant Quantification, Aqueous Kit, Total, BioAssay产品后将其置于4°C/-70°C保存。
其他
Usbiological公司是美国著名的抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品,为科研用户提供了诸多超值选择。
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该页面的中文产品信息的翻译,仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前,请浏览说明书确认。
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