- 中文名称
a1-Antitrypsin, Human AAT, Serpin Peptidase Inhibitor Clade A, SerpinA1) BioAssay ELISA Kit
- 英文名字
- a1-Antitrypsin, Human AAT, Serpin Peptidase Inhibitor Clade A, SerpinA1) BioAssay ELISA Kit
- 供应商
- USBiological
- 产品货号
- A2298-32H
- 产品报价
- ￥1/96Tests
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Alpha 1-Antitrypsin is a 52kD serine protease inhibitor (serpin) in blood, which protects tissue from enzymes from inflammatory cells, especially elastase. In certain acute phase inflammatory reactions, A1AT is elevated in order to limit the damage caused by activated neutrophil granulocytes and their enzyme elastase. Disorders of A1AT include A1AT deficiency, a hereditary disorder that can lead to severe tissue breakdown during inflammation. This may result in pulmonary emphysema and liver cirrhosis, in severe cases. Genetic variants of A1AT do occur.Sample Type:BloodIntended Use:The Alpha 1-Antitrypsin (A1AT) test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring A1AT in biological fluid of humans.Test Principle:The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the Alpha 1-Antitrypsin present in samples reacts with the anti-Alpha 1-Antitrypsin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-A1AT antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound A1AT. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’- tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of A1AT in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of A1AT in the test sample. The quantity of A1AT in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.Kit Components:Diluent concentrate (Running Buffer)One bottle containing 1x50ml of a 5X concentrated diluent running buffer.Wash solution concentrateOne bottle containing 1x50ml of a 20X concentrated wash solution.Enzyme-antybody conjugate 100XOne vial containing 1x150ul of affinity purified anti-Human Alpha 1-Antitrypsin antibody conjugated with horseradish peroxidase in a stabilizing buffer.Chromogen-substrate solution One vial containing 1x12ml of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.Stop solutionOne vial containing 1x12ml 0.3M sulfuric acid.Anti-human A1M ELISA micro plateTwelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Human A1M.Human alpha 1-antitrypsin calibratorOne vial containing a lyophilized Human Alpha 1-Antitrypsin calibrator.Storage and Stability:Store all components at 4°C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- 产品特点
- 针对a1-Antitrypsin, Human AAT, Serpin Peptidase Inhibitor Clade A, SerpinA1) BioAssay ELISA Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到a1-Antitrypsin, Human AAT, Serpin Peptidase Inhibitor Clade A, SerpinA1) BioAssay ELISA Kit产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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