- 中文名称
Angiotensin Converting Enzyme 2 Activity, Fluorometric 390, BioAssay Kit (ACE-2, ACEH)
- 英文名字
- Angiotensin Converting Enzyme 2 Activity, Fluorometric 390, BioAssay Kit (ACE-2, ACEH)
- 供应商
- USBiological
- 产品货号
- A2295-04B
- 产品报价
- ￥1/1Kit
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Angiotensin I converting enzyme 2 (ACE2), the newest member of the renin angiotensin system (RAS), is a zinc metallopeptidase that plays a central role in the control of angiotensin peptides (1,2). ACE2 has direct effects on cardiac function (3), and is expressed predominantly in vascular endothelial cells of the heart and the kidneys (2). It has been reported that ACE2 might protect kidneys in early stages of diabetes (4). In addition to its role in the regulation of hypertension, ACE2 is a functional receptor for coronavirus that causes severe acute respiratory syndrome (SARS) (5). ACE2 is considered an important therapeutic target for controlling cardiovascular diseases, kidney disease and severe acute respiratory syndrome (SARS) outbreaks.The Fluorometric ACE2 Activity Assay Kit provides a convenient assay for high throughput screening of ACE2 inhibitors and inducers and for continuous assay of ACE2 activity using Mca/Dnp fluorescence resonance energy transfer (FRET) peptide. In the FRET peptide the fluorescence of Mca is quenched by Dnp. Upon cleavage into two separate fragments by the enzyme, the fluorescence of Mca is recovered, and can be monitored at excitation/emission = 330/390nm. The assay can detect the activity of subnanogram level of ACE2. Assays are performed in a convenient 96-well microplate format.Kit Components:A2295-04B1: Mca/Dnp, ACE2 Substrate, Ex/Em=330nm/390nm upon cleavage 5mM, 1x50ulA2295-04B2: Mca Fluorescence Reference Standard, Ex/Em=330nm/390nm, 1mM, 1x10ulA2295-04B3: Assay Buffer 1x20mlA2295-04B4: Inhibitor of ACE2, 100uM, 1x10ulA2295-04B5: Stop Solution, 1x10mlStorage and Stability:Store all kit components at -20°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.• Protect A2295-04B1 and A2295-04B2 from light and from moisture.• A2295-04B3 and A2295-04B5 can be stored at room temperature for convenience.Materials Required But Not Provided:• ACE2 source: The active enzyme, cell lysates, tissue extracts.• 96-well microplate: Black microplates provide better signal to noise ratio.• Fluorescence microplate reader: Capable of detecting emission at 390nm with excitation at 330nm.Quality Control:As reported
- 产品特点
- 针对Angiotensin Converting Enzyme 2 Activity, Fluorometric 390, BioAssay Kit (ACE-2, ACEH) 该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Angiotensin Converting Enzyme 2 Activity, Fluorometric 390, BioAssay Kit (ACE-2, ACEH) 产品后将其置于-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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