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中文名称

β-Amyloid (1-42) BioAssay ELISA Kit

英文名字
β-Amyloid (1-42) BioAssay ELISA Kit
供应商
USBiological
产品货号
A2275-75R
产品报价
¥1/1Kit
产品说明书
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产品新闻
背景资料
USBiological品牌是全球有名的抗原抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理,是行业中少有的致力于服务客户,帮助客户,且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应,专业的产品和解决方案支持,稳健快捷的交货周期,优质放心的售后服务。我们致力于为您提供有价值的产品和服务,在意您的成功!
产品描述
Intended Use: The Amyloid (1-42) ELISA is to be used for the in vitro quantitative determination of human b-amyloid 42 in samples (e.g., tissue culture medium, tissue homogenate, cerebrospinal fluid (CSF), etc.). The assay will recognize both natural and synthetic forms of human b-amyloid 42. Anti-human b-amyloid 42 antibody used in this kit is capable of selectively detecting b-amyloid 42 and not b-amyloid 40/ b-amyloid 43.Principle of the Test:A2275-75R is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). A monoclonal antibody specific for the NH2-terminus of human b-amyloid has been coated onto the wells of the microtiter strips provided. Samples, including standards of known human b-amyloid content, control specimens and unknowns are pipetted into these wells, and co-incubated with a rabbit antibody specific for the COOH-terminus of the 1-42 b-amyloid sequence. This COOH-terminal sequence is created upon cleavage of the analyzed precursor. Bound rabbit antibody is detected by the use of a horseradish peroxidase-labeled anti-rabbit antibody. After removal of excess anti-rabbit antibody, a substrate solution is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of human b-amyloid (1-42) present in the original specimen.Kit Components:Microtiter plate, 1x96wells. Plate pre-coated with monoclonal antibody to N-terminus of beta-amyloidb-Amyloid 1-42 Standard, 1x1 vial. Lyophilized synthetic peptide. Refer to vial label for quantity and reconstitution volume.Standard Diluent Buffer, 1x60ml. Contains 15mM sodium azide and red dye.Detection Antibody, 1x6ml. Rabbit anti-human b-amyloid 42. Contains 15mM sodium azide and blue dye.IgG(HRP) (100X), 1x125ul. Anti-rabbit IgG conjugated to horseradish peroxidase. Contains 3.3mM thymol and yellow dye.HRP Diluent, 1x25ml. Contains 3.3mM thymol and yellow dye.TMB, 1x25ml. Stabilized tetramethylbenzidineStop Solution, 1x25mlWash Buffer Concentrate (25X), 1x100mlStorage and Stability:Store kit components at 4°C. Stable for at least 6 months. Store reconstituted human b-amyloid 1-42 standard in aliquots at -70°C for up to 4 months, For maximum recovery of product, centrifuge the original vial prior to removing the cap.
产品特点
针对β-Amyloid (1-42) BioAssay ELISA Kit该产品的特点优势,欢迎查阅官网提供的产品说明书。
保存建议
建议收到β-Amyloid (1-42) BioAssay ELISA Kit产品后将其置于4°C保存。
其他
Usbiological公司是美国著名的抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品,为科研用户提供了诸多超值选择。
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