Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade，and this critical regulatory link functions as a switch determining cell fate decisions including proliferation，differentiation，apoptosis，survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339，by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2，ADCY5 and ADCY6，resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity.
应用类型
Immunofluorescence (IF), Immunocytochemistry (ICC), Western Blotting, ELISA
免疫原
A synthesized peptide derived from human C-RAF around the phosphorylation site of Sersine 301.
