Suppressor of microRNA (miRNA) biogenesis，including that of let-7 and possibly of miR107，miR-143 and miR-200c. Binds primary let-7 transcripts (pri-let-7)，including pri-let-7g and pri-let-7a-1，and sequester them in the nucleolus，away from the microprocessor complex，hence preventing their processing into mature miRNA (PubMed:22118463). Does not act on pri-miR21. The repression of let-7 expression is required for normal development and contributes to maintain the pluripotent state of embryonic stem cells by preventing let-7-mediated differentiation. When overexpressed，recruits ZCCHC11/TUT4 uridylyltransferase to pre-let-7 transcripts，leading to their terminal uridylation and degradation (PubMed:19703396). This activity might not be relevant in vivo，as LIN28B-mediated inhibition of let-7 miRNA maturation appears to be ZCCHC11-independent. Interaction with target pre-miRNAs occurs via an 5'-GGAG-3' motif in the pre-miRNA terminal loop. Mediates MYC-induced let-7 repression. When overexpressed，isoform 1 stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth.
应用类型
Western Blotting
免疫原
Peptide sequence around aa.242~246( P-S-V-Q-K) derived from Human LIN28B
