- 中文名称
AMPK, ACC, Antibody Sampler Kit, BioAssay
- 英文名字
- AMPK, ACC, Antibody Sampler Kit, BioAssay
- 供应商
- USBiological
- 产品货号
- A1475-25
- 产品报价
- ￥1/1Kit
- 产品说明书
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- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- The AMPK and ACC Antibody Sampler Kit provides an economical means to investigate energy homeostasis and fatty acid synthesis within the cell. The kit contains primary and secondary antibodies to perform four Western mini-blots with each antibody. Background: AMP-activated protein kinase (AMPK) is highly conserved from yeast to plants and animals and plays a key role in the regulation of energy homeostasis (1). AMPK is a heterotrimeric complex composed of a catalytic subunit and regulatory and subunits, each of which is encoded by two or three distinct genes ( 1, 2; 1, 2; 1, 2, 3)(2). The kinase is activated by an elevated AMP/ATP ratio due to cellular and environmental stress, such as heat shock, hypoxia and ischemia (1). The tumor suppressor LKB1, in association with accessory proteins STRAD and MO25, phosphorylates AMPK at Thr172 in the activation loop and this phosphorylation is required for AMPK activation (3-5). AMPK is also phosphorylated at Thr258 and Ser485 (for 1; Ser491 for 2). The upstream kinase and biological significance of these phosphorylation events have yet to be elucidated (6). The 1 subunit is post- translationally modified by myristoylation and multi-site phosphorylation including Ser24/25, Ser96, Ser101 and Ser182 (6,7). Phosphorylation at Ser108 of the 1 subunit seems to be required for the activation of AMPK enzyme, while phosphorylation ot Ser24/25 and Ser182 affects AMPK localization (7). Accumulating evidence indicates that AMPK not only regulates the metabolism of fatty acids and glycogen, but also modulates protein synthesis and cell growth through EF2 and TSC2/mTOR pathways, as well as blood flow via eNOS/nNOS (1). Acetyl-CoA carboxylase (ACC) catalyzes the pivotal step of the fatty acid synthesis pathway. The 265kD ACC is the predominant isoform found in liver, adipocytes and mammary gland, while the 280kD ACC is the major isoform in skeletal muscle and heart (8). Phosphorylation by AMPK at Ser79 or by PKA at Ser1200 inhibits the enzymatic activity of ACC (9). ACC is a potential target of anti-obesity drugs (10,11). Kit Components: Vol. Applications MW ReactivityA1475-25A: Phospho-AMPK alpha (Thr172) 20ul W, IHC-P 62kD Rabbit Mab H M R Mk Sc Dm (C) (B) A1475-25B: AMPK alpha 20ul W,IP,F 62kD Rabbit Mab H M R MkA1475-25C: AMPK beta1Phosphorylated (Ser108) 20ul W,IP 38kD Rabbit Pab H M R MkA1475-25D: AMPK beta1/2 20ul W,IHC-P, IF-IC, F 34,38kD Rabbit Mab H M R Mk HmA1475-25E:Acetyl-CoA Carboxylase Phosphorylated (Ser79)20ul W, IP, IHC-P, IF-IC 280kD Rabbit Pab H M R Mk (C) (B) A1475-25F: Acetyl-CoA Carboxylase 20ul W, IHC-P, IF-IC, F 280kD Rabbit Mab H M RA1475-25G: IgG (HRP) 100ul Goat PabRecommended Dilution:Western BlotIHC-PIP IF-ICFA1475-25A1:10001:50A1475-25B1:10001:1001:50A1475-25C1:10001:50A1475-25D1:10001:501:501:100A1475-25E1:10001:4001:1001:25A1475-25F1:10001:251:2001:400Optimal dilutions to be determined by the researcher.Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- 产品特点
- 针对AMPK, ACC, Antibody Sampler Kit, BioAssay该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到AMPK, ACC, Antibody Sampler Kit, BioAssay产品后将其置于-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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