Ammonia is an important source of nitrogen for living systems. Nitrogen is required for thesynthesis of amino acids, which are the building blocks of protein. Ammonia is a metabolicproduct which is created through amino acid deamination. It plays an important role in bothnormal and abnormal animal physiology such as acid/base balance. USBio provides a rapid, simple, sensitive, and reliable assay suitable for research and high throughput assay of Ammonia. In this non-enzymatic assay, ammonia is used to form indophenol, a highly colored product easily quantifiable by colorimetry (OD670nm) using a plate reader. The kit can detect less than 1nmol (~10uM) ammonia using either a 96 well or 384 well microwell plate.Kit Components:Ammonia Reagent 1 1x8mlAmmonia Reagent 2 1x4mlAmmonium Chloride Standard (100mM) 1x0.1ml.Storage and Stability:Store at 4°C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
