Required for disulfide bond formation in some periplasmic proteins such as PhoA or OmpA. Acts by transferring its disulfide bond to other proteins and is reduced in the process. DsbA is reoxidized by DsbB. Required for pilus biogenesis. PhoP-regulated transcription is redox-sensitive,being activated when the periplasm becomes more reducing (deletion of dsbA/dsbB,treatment with dithiothreitol). MgrB acts between DsbA/DsbB and PhoP/PhoQ in this pathway.
应用类型
WB
免疫原
Recombinant E.coli. DsbA protein fragments over expressed in E.coli