- 中文名称
Albumin, Rat, BioAssay ELISA Kit
- 英文名字
- Albumin, Rat, BioAssay ELISA Kit
- 供应商
- USBiological
- 产品货号
- A1274-92B
- 产品报价
- ￥1/96Tests
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- The Rat Albumin ELISA is a highly sensitive two-site enzyme linked immunoassay for measuring Albumin in biological samples of rats.Albumin (Alb) is an amazing polyfunctional protein contributing to homeostasis through mechanisms of hemodynamics, transport and nutrition. Albumin is found both intra- and extravascularly in all mammals and many lower vertebrates. It is a molecule of about 67kD, synthesized by the liver. Normally only very trace amounts of albumin escape reabsorption by kidney glomeruli and is excreted into the urine. Many occult diseases can cause kidney damage which may result in excessive amounts of serum proteins, including albumin, to be excreted by the kidney and into the urine. This ELISA kit can be used to measure albumin in serum, tissue extracts and other biological fluids.Principle of the Assay:In this assay the albumin present in samples reacts with the anti-albumin antibodies which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, anti-albumin antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound albumin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of albumin in the sample tested; thus, the absorbance at 450nm is a measure of the concentration of albumin in the test sample. The quantity of albumin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.Kit Components:A1274-92B1: Microtiter Plate, 1x 96 wells (12 removable 8-microwell strips in well holder frame. Each well is coated with purified anti-rat albumin.A1274-92B2: Antibody (HRP), 1x150ul. Undiluted affinity-purified anti-rat albumin antibody conjugated with horseradish peroxidase in a stabilizing buffer*A1274-92B3: Calibrator, 1x 1vial. Lyophilized rat albumin calibrator.A1274-92B4: TMB Substrate, 1x12ml. 3,3’,5,5’- tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3. A1274-92B5: Diluent Concentrate, 5X, 1x50ml.A1274-92B6: Wash Solution Concentrate, 20X, 1x50ml.A1274-92B7: Stop Solution, 1x12ml. 0.3M sulfuric acid. Warning: Avoid contact with skin.Storage and Stability:Store *A1274-92B3: Calibrator powder and aliquoted liquid at -20°C. Store other components at 4°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对Albumin, Rat, BioAssay ELISA Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Albumin, Rat, BioAssay ELISA Kit产品后将其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
-
- 注意
-
该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
-
