- 中文名称
Albumin, Human, BioAssay ELISA Kit
- 英文名字
- Albumin, Human, BioAssay ELISA Kit
- 供应商
- USBiological
- 产品货号
- A1274-85M
- 产品报价
- ￥1/96Tests
- 产品说明书
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- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- The analytical determination of the protein albumin in urine is important because increased values indicate an increased risk of developing end-stage renal diseases and cardiovascular disease among people with diabetes. Also albumin in urine is a sensitive indicator of renal damage caused by exposure to nephrotoxic substances. The most significant and well-documented of these abnormalities is a subtle increase in the urinary albumin excretion rate, known as mico-albuminuria. Mico-albuminuria is not measurable by conventional techniques for detecting proteinuria. It is believed that mico-albuminuria represents a reversible stage of renal dysfunction, whereas overt proteinuria reflects irreversible disease. Proteinuria typically appears about twenty years after the onset of diabetes, whereas mico-albuminuria can be detected within the first ten years. Mico-albuminuria (30-150ug/min) has been established as a marker predictive of subsequent development of diabetic nephropathy. Periodic monitoring(2-3 times/year) of urinary of albumin levels in the diabetic patient is therefore recommended so that the initial escalation of renal damage can be detected and appropriate treatment regimens can be instituted. Radial immunodiffusion, immunoturbidimetric, immunophelometric method and RIA have been used for the albumin assay in urine. This Micro- albumin Quantitative using microwell competitive ELISA method provides a convenient, sensitive and specific assay for albumin and free of interference from urine specimens.Sample Type:Urine samples with albumin concentrations greater than 100ug/ml should be diluted with the zero standard (standard A) or normal saline and the results obtained should be multiplied by the appropriate dilution factor. This kit has been used to measure albumin in mice transplanted with human tumors and in media from cultured cells (see references below).Intended Use:This albumin ELISA kit is intended for the measurement of albumin in urine samples only. It cannot be used to measure albumin in serum or plasma. Furthermore, it is recommended that the urine samples should not be frozen before the assay.Sensitivity:Based on sixteen replicates determinations of the zero standard, the minimum albumin concentration detectable using this assay is 1ug/ml. The detection limit is defined as the value deviating by 2 SD from the zero standard.Range:Five different patient urine samples (with original albumin concentrations of 50, 77.6, 103, 176, and 327mg/l) were diluted (1:2, 1:5, and 1:10) with the zero standard and their final albumin values determined. The samples showed excellent mean recoveries of about 94% (range 85-109%).Test Principle:Albumin ELISA kit is based on competitive binding of human albumin from urine samples and albumin coated on the microwell plate to the enzyme labeled anti- human albumin antibody. Higher concentrations of albumin in the urine samples result in decreased binding of enzyme (HRP) labeled antibody to the microwell plate. After a washing step, chromogenic substrate is added and color developed. The enzymatic reaction (color) is inversely proportional to the amount of albumin present in the sample. Adding stopping solution terminates the reaction. Absorbance is then measured on a microtiter well ELISA reader at 450nm. and the concentration of albumin in samples and control is read off the standard curve.Kit Components:Human Albumin coated microwell strip plate: 1 X 96 wells Albumin Std. A: 1 X 11mlAlbumin Std. B (2.5ug/ml): 1 X 0.5ml Albumin Std. C (5ug/ml): 1 X 0.5mlAlbumin Std. D (25ug/ml): 1 X 0.5ml Albumin Std. E (50ug/ml): 1 X 0.5mlAlbumin Std. F (100ug/ml): 1 X 0.5mlAlbumin Control (exact value printed on the vial)Anti-Albumin-HRP Conjugate: 1 X 11mlHRP substrate Solution: 1 X 11mlWash buffer (100X): 1 X 10ml, dilute 1:100 with distilled waterStop solution (ready-to-use): 1 X 10mlStorage and Stability:Store liquid at -20°C. Store other components at 4°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- 产品特点
- 针对Albumin, Human, BioAssay ELISA Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Albumin, Human, BioAssay ELISA Kit产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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