Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest，activation of DNA repair and apoptosis in response to the presence of DNA double-strand breaks. May also negatively regulate cell cycle progression during unperturbed cell cycles. Following activation，phosphorylates numerous effectors preferentially at the consensus sequence [L-X-R-X-X-S/T]. Regulates cell cycle checkpoint arrest through phosphorylation of CDC25A，CDC25B and CDC25C，inhibiting their activity. Inhibition of CDC25 phosphatase activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression.
应用类型
WB, IHC, ELISA
免疫原
Synthesized peptide derived from human Chk2 around the phosphorylation site of T68
