Binds to sialic acid-containing receptors on the cell surface，bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization of about two third of the virus particles through clathrin-dependent endocytosis and about one third through a clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2，releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore. The recombinant HA protein of a clade H5N1 virus was used to generate monoclonal antibodies for viral neutralization assays. The HA full-length protein was expressed in insect cells infected with baculovirus carrying the HA gene to ensure that it underwent glycosylation.
应用类型
FC, IF, WB
免疫原
HA full length that was expressed and purified from insect cells
