Serine/threonine-protein kinase B-raf (BRAF) is a member of the Raf family. BRAF mutations are frequent in benign and malignant human tumors. BRAF V600E mutation accounts for the vast majority of BRAF alterations and the mutation induces a conformational change of the activation segment leading to a constitutive kinase activity of BRAF and consecutive phosphorylation of downstream targets. BRAF V600E mutation have been detected in melanoma，papillary thyroid carcinoma，pleomorphic xanthoastrocytomas，Langerhans cell histiocytosis，borderline ovarian cancer，ganglioglioma，colorectal carcinoma，and pilocytic astrocytoma.
