- 中文名称
Akt, Phosphorylated (Ser473) Survival Marker IHC Detection Kit, BioAssay discontinued
- 英文名字
- Akt, Phosphorylated (Ser473) Survival Marker IHC Detection Kit, BioAssay discontinued
- 供应商
- USBiological
- 产品货号
- A1125-20
- 产品报价
- ￥1/1Kit
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Synthetic phosphopeptide corresponding to residues around Ser473 of mouse Akt (KLH-coupled). Detects Akt1 only when phosphorylated at serine 473. Also detects Akt2 andAkt3 only when phosphorylated at equivalent sites. Does not detect Akt phosphorylated at othersites or related kinases such as PKC and p70 S6 kinase.Akt, Phosphorylated (Ser473) Survival Marker IHC Detection Kit, BioAssay is a “ready to use” system designed to detect the activation of Akt in human tissue and cell preparations using immunohistochemistry. The kit utilizes the ABC immunoperoxidase method to detect endogenous levels of phosphorylated Akt protein. Prediluted Phospho-Akt (Ser473) Antibody is bound by a biotinylated secondary antibody. Avidin DH and biotinylated horseradish peroxidase are complexed by mixing defined amounts prior to use. The mixture subsequently binds the secondary antibody. The macromolecular complex is localized by incubation with NovaRed enzyme substrate. The prediluted primary antibody, along with the ABC system, allows the user to consistently examine phosphorylated-Akt localization and offers the highest sensitivity with the lowest background.Akt, also referred to as PKB or Rac, plays a critical role in controlling the balance between survival and apoptosis (1-3). This protein kinase is activated by insulin and various growth and survival factors, and functions in a wortmannin-sensitive pathway involving PI3 kinase (2,3). Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 (4), and by phosphorylation within the carboxyterminus at Ser473. Akt promotes cell survival by inhibiting apoptosis through its ability to phosphorylate and inactivate several targets, including Bad (5). Forkhead transcription factors (6) and caspase-9. PTEN phosphatase is a major negative regulator of the PI3 kinase/Akt signaling pathway (7). LY294002 is a specific PI3 kinase inhibitor (8). One of the essential functions of Akt is the regulation of glycogen synthesis through phosphorylation and inactivation of glycogen synthase kinase-3a and b (9,10). Akt may also play a role in insulin stimulation of glucose transport (9). In addition to its role in survival and glycogen synthesis, Akt is involved in cell cycle regulation by preventing GSK3b-mediated phosphorylation and degradation of cyclin D1 (11), and by negatively regulating the cyclindependent kinase inhibitors p27 KIP (12) and p21 WAF1 (13). Akt also plays a critical role in cell growth by directly phosphorylating the mammalian target of rapamycin, mTOR (14), but more importantly through phosphorylation and inactivation of tuberin (TSC2), an mTOR inhibitor (15). Inhibition of mTOR stops the protein synthesis machinery due to inactivation of its effector, p70 S6 kinase, and activation of the eukaryotic initiation factor, 4E binding protein 1 (4E-EP1), an inhibitor of translation (16,17).Applications: Suitable for use in Immunohistochemistry. Other applications not tested.Purity (antibodies): Purified by Protein A and immunoaffinity chromatography.Quantitity: Sufficient material for 150 slides.Kit Components:Cap ColorPeroxidase QuenchOrangeBlocking SolutionBluePrediluted Phospho-Akt (Ser473) AntibodyPurplePrediluted Negative ControlBrownBiotinylated Secondary AntibodyGreenA and B ReagentsGrayNovaRed Substrate*RedPhospho-Akt (Ser473) Blocking PeptideWhite* NovaRed is a trademark of Vector LabsComponents are ready to use and should not be aliquotted.Blocking Solution, Prediluted Phospho-Akt (Ser473) Antibody, Prediluted Negative Control andBiotinylated Secondary Antibody contain 0.05% sodium azide.Storage and Stability:Storage: Store at 4°C.
- 产品特点
- 针对Akt, Phosphorylated (Ser473) Survival Marker IHC Detection Kit, BioAssay discontinued该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Akt, Phosphorylated (Ser473) Survival Marker IHC Detection Kit, BioAssay discontinued产品后将其置于-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
-
- 注意
-
该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
-
