- 中文名称
Akt, Phosphorylated (S473), BioAssay ELISA Kit
- 英文名字
- Akt, Phosphorylated (S473), BioAssay ELISA Kit
- 供应商
- USBiological
- 产品货号
- A1124-95
- 产品报价
- ￥1/1Kit
- 产品说明书
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- The serine/threonine kinase Akt, also known as protein kinase B (PKB), is a central player in such diverse cellular processes as glucose uptake, cell cycle progression, and apoptosis. In mammals, three highly homologous members define the Akt family: Akt1 (PKBa), Akt2 (PKBb), and Akt3 (PKBg). Akt1 is the most ubiquitously expressed member, while Akt2 is expressed predominantly in insulin target tissues such as liver, skeletal muscle, and fat. Akt3 is expressed most selectively, in brain and testis. All three Akts share a conserved structure: an amino-terminal pleckstrin homology domain to interact with membrane phosphoinositides; a central kinase domain; and a carboxyl-terminal regulatory domain that contains the hydrophobic motif, a hallmark of members of the AGC kinase superfamily. Akt signaling is activated by receptor tyrosine kinases, B- and T-cell receptors, cytokine receptors, and other mechanisms that activate phosphoinositide 3-kinase (PI 3-K). Active PI 3-K generates the second messenger phosphatidylinositol-3,4,5-trisphosphate (PIP3), which recruits Akt to the plasma membrane via the pleckstrin homology domain for subsequent phosphorylation. Phosphoinositide-dependent kinase-1 (PDK-1) phosphorylates Akt in the activation loop of the kinase domain (T308 of Akt1) and an unknown kinase, possibly the rictor-mTOR complex, phosphorylates Akt in the hydrophobic motif (S473 of Akt1) of the regulatory domain. Phosphorylation of both sites is necessary for full Akt activation. Downstream Akt substrates include regulators of metabolism and apoptosis, implicating Akt1-3 as targets for diabetes and cancer therapies.Sample Type:Cell lysatesIntended Use:For the quantitative determination of Akt phosphorylated at S473 in cell lysates.Specificity:Recognizes Akt family members phosphorylated at sites corresponding to S473 of Akt1 from human, mouse, and rat. Specificity was demonstrated using both peptide competition and cross-reactivity analysis.Principle of the Assay:The Akt, phosphorylated (S473) ELISA kit employs a two-site sandwich ELISA to quantitate Akt phosphorylated at S473 in cell lysates. An antibody specific for Akt1, Akt2, and Akt3, binding both phosphorylated and unphosphorylated protein, has been pre-coated onto a microplate. Standards and samples are added and Akt1, Akt2, and Akt3 present are bound by theimmobilized antibody. After washing away unbound material, a biotinylated detection antibody recognizing Akt1 phosphorylated at S473, Akt2 phosphorylated at S474, and Akt3 phosphorylated at S472 is used to detect only phosphorylated protein utilizing a standard streptavidin-HRP format. Substrate Solution is added to the wells and color develops in proportion to the amount of phosphorylated Akt bound in the initial step. The color development is stopped and the intensity of the color is measured.Kit Components:A1124-95A; Microtiter Plate, 12 strips x 8wells. Coated with Phospho-Akt (S473) pan-specific antibody. Return unused wells to the foil pouch containing the desiccant pack, resealalong entire edge of zip-seal. May be stored for up to 1 month at 2-8° C.A1124-95B: Standard (Phospo-Akt (S473), Pan-specific), 2x 185ng. Each vial vial contains lyophilized recombinant phospho-Akt2 in a buffered protein base with preservatives. Use within 1 hour of reconstitution. Use a fresh standard for each assay.A1124-95C: Detection Antibody, 1x1vial. Contains 7.5ug of lyophilized biotinylated mouse anti-phospho-Akt (S473) pan-specific antibody.A1124-95D: Lysis Buffer, 1x21ml. Cell lysing buffer with phosphatase inhibitors and preservatives.A1124-95E: Sample Diluent Concentrate (5X), 1x21 ml. 5-fold concentrated buffer with preservatives.A1124-95F: Reagent Diluent Concentrate (10X), 1x21ml. 10-fold concentrated solution of buffered protein base with preservatives.A1124-95G: Wash Buffer Concentrate (25X), 1x21ml. 25-fold concentrated solution of buffered yellow surfactant with preservative. May turn yellow over time.A1124-95H: Color Reagent A, 1x12.5ml. Stabilized hydrogen peroxide.A1124-95I: Color Reagent B, 1x12.5ml Stabilized chromogen (tetramethylbenzidine).A1124-95J: Streptavidin-HRP, 1x1ml. Streptavidin conjugated to horseradish peroxidase.A1124-95K: Stop Solution, 1x6ml. 2N sulfuric acid.Storage and Stability:Store all kit components at 4°C.
- 产品特点
- 针对Akt, Phosphorylated (S473), BioAssay ELISA Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Akt, Phosphorylated (S473), BioAssay ELISA Kit产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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