PARP，a 116 kDa nuclear poly (ADP-ribose) polymerase，appears to be involved in DNA repair in response to environmental stress. This protein can be cleaved by many ICE-like Caspases in vitro and is one of the main cleavage targets of caspase-3 in vivo. In human PARP，the cleavage occurs between Asp214 and Gly215，which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis.
应用类型
WB
免疫原
A synthetic peptide corresponding to residues before the cleavage site of human PARP-1 was used as immunogen. This antibody recognizes full-length form of PARP and PARP-1 only.
