- 中文名称
Aggrecan, PG, Human, BioAssay ELISA Kit (Proteoglycan, PG, AGC 1, Aggrecan 1, Antigen identified by monoclonal A0122, Cartilage specific proteoglycan core protein, Chondroitin sulfate proteoglycan cor
- 英文名字
- Aggrecan, PG, Human, BioAssay ELISA Kit (Proteoglycan, PG, AGC 1, Aggrecan 1, Antigen identified by monoclonal A0122, Cartilage specific proteoglycan core protein, Chondroitin sulfate proteoglycan core protein 1, CSPCP, CSPG1, CSPGCP, Large aggregating proteoglycan, MSK16, SEDK)
- 供应商
- USBiological
- 产品货号
- A1059-52
- 产品报价
- ￥1/96Tests
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Assay for quantitative determination of human Aggrecan PG.Sensitivity: 0.9ng/mLRange: ~10-250ng/mLIncubation Time: 3h 25minSamples: Synovial fluid, serum and culture mediaPrinciple of the test:The Aggrecan, Human, BioAssay ELISA Kit is a solid phase ELISA performed on microtiter plate. The assay is based on an oligoclonal system in which a blend of monoclonal antibodies (MAbs) directed against distinct epitopes of PG are used. Antibody-producing cells are immortilized using the myeloma cell fusion method of Kohler and Milstein. A hybridoma cell is produced which secretes specific homogeneous antibodies. The use of a number of distinct MAbs avoids hyperspecificity and allows highly sensitive assays with extended standard range and short incubation time. Standards or samples containing PG react with capture monoclonal antibodies (MAbs 1) coated on the microtiter well and with a monoclonal antibody (MAb 2) labeled with horseradish peroxidase (HRP). After an incubation period allowing the formation of a sandwich; coated MAbs 1-PG-MAb 2-HRP, the microtiter plate is washed to remove unbound enzyme labeled antibodies. Bound enzyme-labeled antibodies are measured through a chromogenic reaction. Chromogenic Solution TMB+H2O2) is added and incubated. The reaction is stopped with the addition of Stop Solution (HCl) and the microtiter plate is then read at the appropriate wavelength. The amount of substrate turnover is determined colorimetrically by measuring the absorbance which is proportional to the PG concentration. A standard curve is plotted and PG concentrations in a sample is determined by interpolation from the standard curve. The use of the EASIA Reader linearity up to 3OD units) and a sophisticated data reduction method (polychromatic data reduction) result in high sensitivity in the low range and in an extended standard range.Kit Components:A1059-52A: Microtiter Plate 1x96 wells*A1059-52B: Standard 0ng 1x500ul*A1059-52C: Standard 15.7ng 1x500ul *A1059-52D: Standard 28.8ng 1x500ul *A1059-52E: Standard 51.0ng 1x500ul *A1059-52F: Standard 89.0ng 1x500ul *A1059-52G: Standard 197.0ng 1x500ul A1059-52H: Diluent Buffer 2x30mlA1059-52J: Incubation buffer 1x11mlA1059-52K: PG Mab (HRP) 1x5.5ml A1059-52L: Conjugate Buffer 2x11ml*A1059-52M: Control 1 1x108±36ng/ml *A1059-52N: Control 2 1x78±23g/ml *A1059-52P: Control 3 1x38±11ng/ml A1059-52Q: Wash Buffer Concentrate 1x10 mlA1059-52R: Chromogenic Solution TMB (Tetramethylbenzidine) 1x25mlA1059-52S: Stop Solution 1x25mlStorage and Stability:Store *Standards and controls powder at 4°C, liquid aliquots for 2 months at -20°C and 6 months at -70°C. Store other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Immunoassay Control:Ready for use Standard Aggrecan purified from human articular cartilage (A1D1D1 fraction chromatographed on Sepharose CL 2B, material eluted at 0.1 < Kd < 0.7).Controls come with two parameter vials (one high, one low) and are for human markers only.The control set consists of a human aggregan protein, purified from articular cartillage in two different concentrations and provided as lyophilized powder in two different concentrations.
- 产品特点
- 针对Aggrecan, PG, Human, BioAssay ELISA Kit (Proteoglycan, PG, AGC 1, Aggrecan 1, Antigen identified by monoclonal A0122, Cartilage specific proteoglycan core protein, Chondroitin sulfate proteoglycan core protein 1, CSPCP, CSPG1, CSPGCP, Large aggregat该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Aggrecan, PG, Human, BioAssay ELISA Kit (Proteoglycan, PG, AGC 1, Aggrecan 1, Antigen identified by monoclonal A0122, Cartilage specific proteoglycan core protein, Chondroitin sulfate proteoglycan core protein 1, CSPCP, CSPG1, CSPGCP, Large aggregating proteoglycan, MSK16, SEDK)产品后将其置于4°C/-70°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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