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中文名称

Aflatoxin B1, Food, BioAssay ELISA Kit

英文名字
Aflatoxin B1, Food, BioAssay ELISA Kit
供应商
USBiological
产品货号
A0925-08
产品报价
¥1/1Kit
产品说明书
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产品新闻
背景资料
USBiological品牌是全球有名的抗原抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理,是行业中少有的致力于服务客户,帮助客户,且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应,专业的产品和解决方案支持,稳健快捷的交货周期,优质放心的售后服务。我们致力于为您提供有价值的产品和服务,在意您的成功!
产品描述
Aflatoxins belong to the class of mycotoxins. Chemically they are defined as difuranocyclopentanocumarines or difuranopentanolidocumarines, i.e. aflatoxins contain a dihydrofuran or a tetrahydrofuran ring, to which a substituted cumarin system is condensed. Out of about 20 known aflatoxins, the molds Aspergillus flavus and A. parasiticus produce exclusively aflatoxin B1, B2, G1 and G2, and all the other aflatoxins are derivates of these four. The derivates are developed either by metabolism in humans, animals and microorganisms or by environmental reactions.Aflatoxins belong to the strongest mycotoxins, which act primarily in a hepatotoxic and carcinogenic way. The four main aflatoxins show a different toxicity. B1 is without doubt the most toxic aflatoxin, followed by G1, B2 and G2. Aflatoxin B1, however, does not show a direct toxic action. In the process of biotransformation in the liver, the lipophilic toxin is epoxidated and transformed into an active derivative, the so-called aflatoxin B1-2, 3-epoxid. This highly reactive epoxid is able to react with nucleophilic regions of macromolecules. Amongst other this metabolite of aflatoxin B1 binds covalently to the N-7 atom of the guanine bases of DNA. This covalent bond causes an inhibition of the DNA replication, the RNA synthesis and mutations.Both chronical and acute intoxications are effected by aflatoxins. There are only few documented reports about acute intoxications, which are caused by uptake of mycotoxins. Of special importance for human beings are the chronical intoxications by aflatoxins. To the diseases, which develop after such chronical intoxications, belong primary liver carcinoma, hepatitis, Reye’s syndrome and Kwashiorkor. Besides the generation of primary liver carcinoma, aflatoxins are presumably also responsible for other sorts of tumors, like intestinal cancer.Contaminations with aflatoxins occur mostly with nuts and grain. In most cases aflatoxins penetrate the human body via the food. Aflatoxins are stable to heat and are only partly destroyed by boiling. In order to protect people against aflatoxin-induced diseases, there is a need for the quantitative and qualitative control of endangered foodstuff, besides appropriate hygienic precautions, which avoid the formation of aflatoxins. The United States Biological Aflatoxin B1 ELISA is a quick, economical and sensitive method to detect aflatoxin B1 in food. After an appropriate sample preparation, 40 samples can be tested in duplicate within 140 minutes.Intended Use: For the quantitative determination of Aflatoxin B1 in food.Sensitivity: 5pg/mlRecovery (Spiked Samples): ~80%Incubation Time: 140 minKit Components:A0925-08A: Microtiter plate 1x96 wells each, coated with aflatoxin conjugateA0925-08B: Standard 10X (0pg/ml), 1x500ul each in methanolA0925-08C: Standard 10X (100pg/ml), 1x500ul each in methanolA0925-08D: Standard 10X (400pg/ml), 1x500ul each in methanolA0925-08E: Standard 10X (1000pg/ml), 1x500ul each in methanolA0925-08F: Standard 10X (4000pg/ml), 1x500ul each in methanolA0925-08G: Standard 10X (10,000pg/ml), 1x500ul each in methanolA0925-08H: Anti-Aflatoxin B1 Antibody (rabbit), 1x6ml, ready to useA0925-08J: Pab gG (HRP), 1x15ml, ready to useA0925-08K: Substrate Solution (TMB) 1x15ml, ready to useA0925-08L: Stop Solution (0.5M H2SO4), 1x15ml, ready to useA0925-08M: Sample/Standard Diluent (PBS), 1x60ml, ready to useA0925-08N: Wash Solution, 10X (PBS + Tween 20), 1x60ml; Dilute 1:9 with distilled water. If during the cold storage crystals precipitate, the concentrate should be warmed up to 37°C for 15 minutes.Storage and Stability: Store components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
产品特点
针对Aflatoxin B1, Food, BioAssay ELISA Kit该产品的特点优势,欢迎查阅官网提供的产品说明书。
保存建议
建议收到Aflatoxin B1, Food, BioAssay ELISA Kit产品后将其置于4°C保存。
其他
Usbiological公司是美国著名的抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品,为科研用户提供了诸多超值选择。
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