- 中文名称
ADP/ATP Ratio Assay Kit, BioAssay
- 英文名字
- ADP/ATP Ratio Assay Kit, BioAssay
- 供应商
- USBiological
- 产品货号
- A0901-76A
- 产品报价
- ￥1/96Tests
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Changes in the ADP/ATP ratio have been used to differentiate modes of cell death and viability. Increased levels of ATP and decreased levels of ADP signify proliferating cells. Conversely, decreased levels of ATP and increased levels of ADP represent apoptotic or necrotic cells where the decrease in ATP and increase in ADP is much more pronounced in necrosis versus apoptosis.ADP/ATP Ratio Assay Kit provides a rapid method to measure ADP and ATP levels for the screening of apoptosis, necrosis and cell proliferation in mammalian cells. The assay involves two steps. In the first step, the working reagent lyses cells to release ATP and ADP. In the presence of luciferase, ATP immediately reacts with the Substrate D-luciferin to produce light. The light intensity is a direct measure of intracellular ATP concentration.Luciferase ATP+D-luciferin+O2oxyluciferin+AMP+PPi+CO2+lightIn the second step, the ADP is converted to ATP through an enzyme reaction. This newly formed ATP then reacts with the D-luciferin as in the first step.This non-radioactive, homogeneous cell-based assay is performed in microplates. The reagent is compatible with all culture media and with all liquid handling systems for high-throughput screening applications in 96-well and 384-well plates.Key Features:Safe. Non-radioactive assay.Homogeneous and convenient. Mix-incubate-measure type assay.No wash and reagent transfer steps are involved.Robust and amenable to HTS: Z’ factors of 0.5 and above are routinely observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.Applications:Apoptosis and Necrosis determination in cells.Cell proliferation: effects of cytokines, growth factor, nutrients.Drug discovery: high-throughput screening for anticancer drugs.Kit Contents:Assay Buffer: 10mlSubstrate: 120ulCosubstrate 120ulATP Enzyme: 120ulADP Enzyme: 120ulStorage conditions: store all reagents at -20°C. This product is shipped on dry ice. Shelf life: 6 months after receipt.Precautions: reagents are for research use only. Normal precautions for laboratory reagents should be exercised while using the reagents. Please refer to Material Safety Data Sheet for detailed information.Assay Procedure:Assays can be carried out in a tube or in a 96-well plate. For consistency, it is recommended that the time between the three luminescence measurements be the same for all samples.1. Sample Preparation. For suspension cells, transfer 10ul of the cultured cells (103-104) into a white opaque 96 well plate. Adherent cells: culture 103-104 cells in white opaque microplate. At the time of assay, remove the culture medium immediately before adding 100ul ATP Reagent (see below).2. ATP Assay. Bring Assay Buffer, Substrate and Cosubstrate to room temperature. Thaw enzyme on ice or at 4°C. Fresh Reconstitution is recommended. Store unused reagents including the enzyme at -20°C. ATP Reagent. For each 96-well, mix 95ul Assay Buffer with 1ul Substrate, 1ul Cosubstrate and 1ul ATP Enzyme. Add 90ul ATP Reagent to each well and mix by tapping the plate. After 1 min, read luminescence (RLU A) on a luminometer.3. ADP Assay. Prepare ADP Reagent: for each 96-well, mix 5ul dH2O with 1ul ADP Enzyme. Ten minutes after reading the luminescence for ATP (RLU A), read the luminescence of the samples again (RLU B). This measurement provides the background prior to measuring ADP (i.e. the residual ATP signal). Immediately following reading RLU B, add 5ul ADP Reagent to each well and mix by tapping the plate or pipetting up and down. After 1 min. read luminescence (RLU C) on a luminometer.4. Calculation: of ADP/ATP Ratio. Subtract RLU B from RLU C, then divide by RLU A:ADP/ATP Ratio =RLU C–RLU BRLU AResults Interpretation:The interpretation of different ratios obtained may vary significantly according to the cell types and conditions used. However, the following may be used as guidelines:1. Test gives markedly elevated ATP levels with no significant increase in ADP levels in comparison to control cells=proliferation.2. Test gives lower ATP levels with an increase in ADP levels in comparison to control cells=apoptosis.3. Test gives markedly lower ATP levels with greatly increased ADP levels in comparison to control cells=necrosis.
- 产品特点
- 针对ADP/ATP Ratio Assay Kit, BioAssay该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到ADP/ATP Ratio Assay Kit, BioAssay产品后将其置于-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
-
- 注意
-
该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
-
