MAb 113-1 recognizes a 60kDa antigen associated with the mitochondria in human cells. It can be used to stain mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells. This antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells.
Abundant granular eosinophilic cytoplasm is a common feature of renal oncocytoma, chromophobe renal cell carcinoma, eosinophilic variant of papillary renal cell carcinoma, and the granular variant of clear cell renal cell carcinoma (RCC). The chief reason for their distinction from one another is the difference in their biologic behavior. However, precise characterization may be difficult in some cases because of overlapping morphologic features. Immunostaining pattern with anti-mitochondrial MAb 113-1 has been reported as a useful discriminatory adjunct in the complex differential diagnosis of granular renal cell tumors.
Moreover, Salivary gland tumors usually show great variability both in their morphopathology as well as their clinical behavior. One study highlights the usefulness of 113-1 MAb to facilitate the classification of salivary tumors, an aspect that may sometimes have not only diagnostic implications, but also prognostic.
