- 中文名称
Nicotinic Acetylcholine Receptor
- 英文名字
- Nicotinic Acetylcholine Receptor
- 供应商
- Abgent
- 产品货号
- A-AG1421
- 产品报价
- ￥3025/25 ul
- 产品说明书
- 点击查看
- 购买方式
- 产品新闻
- 背景资料
- Acetylcholine, released by cholinergic neurons, activates two groups of acetylcholine receptors (AChRs); muscarinic AChRs (mAChRs) which belong to the superfamily of G-protein coupled receptors (GPCRs) and nicotinic AChRs (nAChRs) which belong to the ligand-gated ion channel superfamily. nAChRs also respond to nicotine, hence their name1. These channel receptors are usually non-selective cation channels activated upon ligand binding which ultimately leads to the depolarization of postsynaptic cell membranes.
To date, 17 different but related subunits of nAChRs have been identified and cloned. They consist of a subunits (α1-10), which is responsible for the binding of ligands. In fact, this subunit includes a Cys-loop in the first extracellular domain that is required for agonist binding2. The other subunits responsible for making up the active receptor are the β (β1-4), γ, δ and ε subunits3. Structurally, all subunits have the following: a conserved large extracellular N-terminal domain, 3 conserved transmembrane domains, a variable cytoplasmic loop and a fourth transmembrane domain with a short extracellular C-terminal domain. An active nAChR is generally a heteropentamer (homopentamers also exist) of these various subunits organized around a central pore1. However, the α7 subunit mainly forms homomeric functional structures, although functional channels have been observed with its association with α5, β2 or β3 subunits1.
Interestingly, the α7 nAChR is the only subunit to be activated by two endogenous ligands: acetylcholine and choline1.
All α subunits are expressed in neuronal cells except for the α1 subunit which is specifically expressed in skeletal muscle. They are also expressed in non-neuronal cells such as bronchial epithelial cells4, as well lymphocytes5. The diversity of these receptors and their functional organization gives rise to unique properties and functions. The α4β2 receptor composition makes up a high affinity nicotinic receptor. In fact, its upregulation (mainly expressed by the increase of functional receptors at the membrane and not expression per se) is responsible for the increased appearance of binding sites following nicotine administration1,3.
α7 nAChR seems to be involved in the impairment of sensory gating in schizophrenic individuals. Indeed, many polyphormisms have been detected in the gene promoter of the receptor6. There is also an association of the receptor with nociception as it, along with α2 and α10 subunits are expressed in DRGs, the nociceptive center7.
Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat Nicotinic Acetylcholine Receptor α7 (nAChRα7). Anti-Nicotinic Acetylcholine Receptor α7 (extracellular) antibody (#AG1421) can be used in western blot, immunohistochemical, immunocytochemical and indirect flow cytometry applications. It has been designed to recognize nAChRα7 from mouse, rat and human samples.
- 应用类型
- WB
- 免疫原
- Q05941
- 来源宿主
- 反应性
- 保存建议
- 其他
- ABGENT
- 注意
-
该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
-
