- 中文名称
TRPM6 (胞外)抗体
- 英文名字
- TRPM6 (extracellular) Antibody
- 供应商
- Abgent
- 产品货号
- A-AG1342
- 产品报价
- ￥4125/50 ul
- 产品说明书
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- 购买方式
- 产品新闻
- 背景资料
- Transient receptor potential (TRP) channels are relatively non-selective ion channels enabling the exchange of cations down their electrochemical gradient. This exchange enables the intracellular rise in Na+ and Ca2+ concentration and ultimately in the cell membrane depolarization, important for action potential propagation and muscle contraction1. They are activated by an extremely broad range of stimuli namely, temperature, voltage, pH, endocrine factors as well as signaling molecules2.
The TRP channel family is composed of 28 members divided in 7 subgroups: TRPV, TRPC, TRPM, TRPA, TRPN, TRPP and TRPML. All members of the TRP family have 6 transmembrane (TM) domains, and a pore domain between the fifth (S5) and sixth (S6) transmembrane domains. In general, TRP channels enable the passage of either Na+ or Ca2+ ions with little or no preference. However, some channels do exhibit some selectivity. Also, TRP channels do not display the positive charges in the S4 voltage-sensing domain like most voltage sensitive channels, although they do display voltage dependency3. In addition, TRP channels have in the C-terminal intracellular region to the S6 domain a TRP domain comprising 25 amino acids that is more or less conserved among most TRP channels. Within the TRP domain, there is a TRP box composed of six amino acids, and TRP box 2 – a proline rich domain1,3. The TRP domain seems to be responsible for the binding of PIP2, a phospholipid important for the regulation of channel activity4.
The TRPM subfamily consists of 8 members divided in to three major groups: TRPM1/3, TRPM4/5, and TRPM6/7. TRPM2 and TRPM8 are not included in any of the groups since they are quite different even with respect to each other. TRPM2, TRPM6 and TRPM7 are distinctly different from the other TRP channels and other ion channels as all three have an active kinase domain in the C-terminal tail. TRPM6 kinase domain is able to phosphorylate TRPM7 thus increasing TRPM7 channel activity. However, TRPM7 cannot phosphorylate TRPM65; it can though autophosphorylate and phosphorylate other proteins1,6. The significance of the kinase activity of these two channels has yet to be determined. Both channels are regulated by cytosolic Mg2+. Extracellular Mg2+ inhibits the channels. Both TRPM6 and TRPM7 are thus important regulators of Mg2+ homeostasis7. TRPM7 does so in a more or less ubiquitous manner whereas TRPM6 does so selectively in the kidney and intestines where it is selectively expressed. Indeed, mutations in TRPM6 results in hypomagnesemia with secondary hypocalcemia8-10. TRPM6 and TRPM7 are able to form homo- and heterodimers, where every channel-type formed demonstrates different properties related to cation affinity, and pH sensitivity7.
Alomone Labs is pleased to offer a highly specific antibody directed against an the 1st extracellular loop of mouse TRPM6. Anti-TRPM6 (extracellular) antibody (#AG1342) can be used in western blot analysis, and has been designed to recognize TRPM6 from mouse, rat and human samples.
- 应用类型
- WB
- 免疫原
- Q8CIR4
- 来源宿主
- 反应性
- 保存建议
- 其他
- ABGENT
- 注意
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