- 中文名称
Two Pore Calcium Channel Protein 2 (胞外)抗体
- 英文名字
- Two Pore Calcium Channel Protein 2 (extracellular) Antibody
- 供应商
- Abgent
- 产品货号
- A-AG1331
- 产品报价
- ￥4125/50 ul
- 产品说明书
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- 购买方式
- 产品新闻
- 背景资料
- Among various vertebrate species, three genes are known to encode two-pore segment channels (TPCs) termed TPC1-3. Interestingly TPC3 seems to be absent from the genomes of primates and rodents1. The primary sequence of these channels indicates the presence of two putative pore-forming repeats. Each repeat contains six transmembrane domains and a pore loop, a structure strikingly reminiscent of many voltage-gated Na+ (Nav) and Ca2+ (Cav) channels2. These twelve transmembrane structures are further thought to form functional dimers3. Both TPC1 and TPC2 show ubiquitous expression, while that of TPC1 is exceptionally high in spleen, lung, liver, and kidney2.
Ca2+-mobilizing messengers such as inositol triphosphate, cyclic ADP ribose and nicotinic acid adenosine dinucleotide phosphate (NAADP) are responsible for the intracellular changes in Ca2+ ion concentration4.
In contrast to the other Ca2+-mobilizing agents, NAADP, the most potent of these Ca2+ releasing molecules increases the cytosolic Ca2+ concentration via Ca2+ channels located on acidic vesicles (endolysosomes)5,6. Only quite recently, after almost a decade of being cloned, TPC1 and TPC2 were both found to be responsible for the NAADP-induced release of Ca2+ 7,8. Evidence that these two channels are indeed responsible for the release of Ca2+ is quite compelling since overexpression of TPC1 and its knockdown or point mutation of a critical residue increase and exacerbate Ca2+ release respectively7. In addition, b-cells from TPC2 knockout mice exhibited no Ca2+ release from endolysosomes upon NAADP stimulation8. Finally, in a study using immunopurified channels, it was demonstrated that TPC1 and TPC2 both respond to very low concentrations of NAADP and are unequivocally responsible for the release of Ca2+, whereas TPC3 may negatively regulate the release of Ca2+ 9.
As these channels have only recently been discovered, very little is known about their physiology and gating mechanisms. Their probable involvement in a number of diseases such as lysosomal storage disease (LSDs), caused by the dysfunction of lysosomal associated proteins, has yet to be deciphered1.
Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of mouse Two Pore Calcium Channel Protein 2. Anti-Two Pore Calcium Channel Protein 2 (extracellular) antibody (#AG1331) can be used in western blot analysis, and has been designed to recognize Two Pore Calcium Channel Protein 2 from mouse, rat and human samples.
- 应用类型
- WB
- 免疫原
- Q8BWC0
- 来源宿主
- 反应性
- 保存建议
- 其他
- ABGENT
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