DHR is a cell-permeable fluorogenic probe that is used as an indicator of intracellular peroxynitrite formation. It is oxidized by peroxynitrite to the highly fluorescent product rhodamine in vitro. Neither nitric oxide， superoxide， nor hydrogen peroxide alone appear to oxidize DHR. Formation of rhodamine can be monitored by fluorescence spectroscopy using excitation and emission wavelengths of 500 and 536 nm， respectively， or by absorbance spectroscopy at 500 nm （ε = 78，800 M-1cm-1）. DHR has been used to investigate reactive oxygen intermediates produced by endothelial cells， eosinophils， and reactive microglia.
产品描述
A fluorophore that can be used as an indicator of peroxynitrite formation； neither NO， superoxide， nor hydrogen peroxide alone appear to oxidize DHR； used to investigate reactive oxygen intermediates produced by endothelial cells， eosinophils， and reactive microglia
