L-Arginine serves as a common substrate for both nitric oxide synthase （NOS） and arginase in the cell. NOS catalyzes the oxidation of arginine to citrulline and NO with N.omega.-hydroxy-L-arginine （NOHA） formed as an intermediate. Arginase， on the other hand， catalyzes the hydrolysis of arginine into urea and L-ornithine. Thioarginine （hydrobromide） is a colorimetric substrate for arginase that provides the basis for continuous spectrophotometric measurement of enzyme activity. It exhibits Km and kcat values of 0.5 mM and 18，000 min-1， respectively， which are similar to physiological substrate arginine. The thiol product of the reaction reacts with DTNB to produce 2-nitro-5-thiobenzoate for easy visualization at 405-420 nm.
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Thioarginine （hydrobromide） is a colorimetric substrate for arginase that provides the basis for continuous spectrophotometric measurement of enzyme activity.{9082} It exhibits Km and kcat values of 0.5 mM and 18，000 min-1， respectively， which are similar to physiological substrate arginine.{9082} The thiol product of the reaction reacts with DTNB to produce 2-nitro-5-thiobenzoate for easy visualization at 405-420 nm.
