中文名称

【p70 S6K】p70 S6K蛋白，有活性的（5 ug）

英文名字

p70 S6K, Active

供应商

Biovision

产品货号

7725-5

产品报价

￥已停产

产品说明书

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购买方式

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产品新闻

背景资料

Activation of cell growth leads to the multiple phosphorylation of 40S ribosomal protein S6. The kinase responsible for controlling this event is termed p70S6K (1). Northern blot analysis shows p70S6K to be ubiquitously expressed in human adult tissues. p70S6K is activated by serum stimulation, and the serum-induced activation is inhibited by wortmannin and rapamycin. p70S6k activity undergoes changes in the cell cycle and increases 20-fold in G1 cells released from G0 (2). The kinase is reactivated 10-fold when cells released from a nocodazole-induced metaphase block enter G1 of the next cell cycle. The immunosuppressive agent rapamycin induces inactivation of p70s6k with no effect on other mitogen-activated kinases (3). The principal target of rapamycin-induced p70s6k inactivation is T389 site on p70S6K, which is located in an unusual hydrophobic sequence outside the catalytic domain. Mutation of T389 to alanine ablates kinase activity, whereas mutation to glutamic acid confers constitutive kinase activity and rapamycin resistance. p70S6K activation by growth factor requires phosphorylation by various inputs on multiple sites (4). p70S6K activation requires sequential phosphorylations at proline-directed residues in the putative autoinhibitory pseudosubstrate domain, as well as threonine 389. Threonine 229, a site in the catalytic loop is phosphorylated by phosphoinositide-dependent kinase 1 (PDK-1). Activation of p70S6K requires a phosphoinositide 3-kinase (PI3-K)-dependent signal(s).

序列

p70 S6K蛋白,有活性的蛋白产品，又称p70, Ribosomal protein S6 kinase beta-1, Serine/threonine-protein kinase 14A.其对应NCBI基因号为2268，NCBI基因别称为FGR源，对应Accession号为P09769.该蛋白产品又称p70s6k, p70-s6k, Active p70 S6K, p70 S6K, human p70 S6K, active kinase, active kinases, recombinant p70 S6K, recombinant p70 S6K, kinase activity assay, protein kinase等.

来源宿主

提供的【p70 S6K】p70 S6K蛋白,有活性的产品的来源为Sf9昆虫细胞-杆状病毒中重组表达.

溶解建议

提供的p70 S6K蛋白,有活性的 产品为液态形式溶解于储存液(50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% 甘油)中.

保存建议

在您收到艾美捷发出的【p70 S6K】p70 S6K蛋白,有活性的产品后，建议将其置于-80°C保存.

其他

BioVision的公司总部在美丽的旧金山湾地区，它是全球生命科学公司的Leader，BioVision在开发和研究细胞凋亡检测分析、代谢检测方面具有卓越的影响力.包括检测各种检测分析试剂盒、相关特色抗体、重组蛋白及细胞因子和酶等多种产品，以及其他创新的研究工具.其产品覆盖细胞凋亡研究、能量代谢、细胞/组织蛋白提取、氧化应激与损伤、代谢与肥胖症、细胞增殖与毒性、信号转导、干细胞研究等领域.作为多家知名品牌的中国总代理以及中国区域代理，艾美捷与BioVision一起为研究者们提供优质、新的细胞生物学研究工具而不断扩大产品及服务质量.

注意

该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。

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