Programmed cell death protein 1 (PD-1) is also known as CD279 and PDCD1，is a type I membrane protein and is a member of the extended CD28/CTLA-4 family of T cell regulators. PDCD1 is expressed on the surface of activated T cells，B cells，macrophages，myeloid cells and a subset of thymocytes. PD-1 has two ligands，PD-L1 and PD-L2，which are members of the B7 family. PD-L1 is expressed on almost all murine tumor cell lines，including PA1 myeloma，P815 mastocytoma，and B16 melanoma upon treatment with IFN-γ. PD-L2 expression is more restricted and is expressed mainly by DCs and a few tumor lines. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1，IL-4，IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition，coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. In vitro，treatment of anti-CD3 stimulated T cells with PD-L1-Ig results in reduced T cell proliferation and IFN-γ secretion.
应用类型
WB: 1-2 µg/ml, ELISA: 0.2-1 µg/ml.
免疫原
Human CellExp™ PD-1 /PDCD1, human recombinant (Cat # 7498)
来源宿主
兔
反应性
经检测验证，可与人种属来源的PD-1/PDCD1蛋白 发生免疫反应.
保存建议
提供的PD-1/PDCD1抗体 为无色溶液形式，产品浓度为与批次相关，溶解于包含有100 mM glycine与海藻糖的50 mM Tris缓冲液（pH 7.0）.请在收到该PD-1/PDCD1抗体 产品后，将其置于-20 °C冷冻保存，建议分装使用以避免反复冻融.
