Angiopoietin-1 （Ang-1） is a secreted ligand for Tie-2，a tyrosine-kinase receptor expressed primarily on vascular endothelial cells and early hematopoietic cells. Ang-1/ Tie-2 signaling promotes angiogenesis during the development，remodeling，and repair of the vascular system. Transgenic mice lacking expression of either Ang-1 or Tie-2 fail to develop a fully functional cardiovascular system and die before birth. Postnatally，the angiogenic activity of Ang-1/Tie-2 is required during normal tissue repair and remodeling of the female endometrium in the menstrual cycle. Ang-1/Tie-2 signaling appears to be regulated by Angiopoietin-2 （Ang-2），a natural antagonist for Tie-2 that exerts its effects through an internal autocrine loop mechanism. In addition to suppressing endothelial cell activation by inhibiting the expression of adhesion and inflammatory molecules，Ang-1 enhances endothelial cell survival and capillary morphogenesis，and lessens capillary permeability. As such，Ang-1 has a potential to become an effective therapeutic agent for treating various endothelium disorders，including several severe human pulmonary diseases. The efficacy of cell-based Ang-1 gene therapy for acute lung injury （ALI） has recently been studied in a rat model of ALI. The results of this study show that such therapy can markedly improve lung condition and suggest that Ang-1 therapy may represent a potential new strategy for the treatment and/or prevention of acute respiratory distress injury （ARDI），a significant cause of morbidity and mortality in critically ill patients. Recombinant human ANG-1，derived from HeLa cells，is a C-terminal histidine tagged glycoprotein which migrates with an apparent molecular mass of 60.0 – 70.0 kDa by SDS-PAGE under reducing conditions. Sequencing analysis shows N-terminal sequences starting with Ser-20 and with Asp-70 of the 498 amino acid precursor protein.
应用类型
Western blot: ~1:1000.
免疫原
This ANGPT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 374-402 amino acids from the C-terminal region of human ANGPT1.
