PARP，a 116 kDa nuclear poly （ADP-ribose） polymerase，appears to be involved in DNA repair in response to environmental stress. This protein can be cleaved by many ICE-like Caspases in vitro and is one of the main cleavage targets of caspase-3 in vivo. In human PARP，the cleavage occurs between Asp214 and Gly215，which separates the PARP amino-terminal DNA binding domain （24 kDa） from the carboxy-terminal catalytic domain （89 kDa）. PARP helps cells to maintain their viability； cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis.
应用类型
WB: 1 ug/ml， IHC: 8-25 ug/ml.
免疫原
E. coli-derived recombinant human PARP- Thr 373-Glu 540
