Ubiquitinating enzymes (UBEs) catalyze protein ubiquitination，a reversible process countered by deubiquitinating enzyme (DUB) action. Five DUB subfamilies are recognized，including the USP，UCH，OTU，MJD，and JAMM enzymes. The deubiquitinating enzyme ubiquitin-specific protease 8 (USP8/UBPy) is a cysteine protease belonging to the USP/UBP subfamily. Research studies have shown that USP8 is an essential growth-regulated enzyme indispensible for cell proliferation and survival. Indeed，conditional knock-out of murine USP8 was shown to promote a dramatic loss in expression of receptor tyrosine kinases，including EGFR，ErbB3，and c-Met. In agreement with these findings，USP8 inactivation leads to enhanced ubiquitination of ligand-activated EGFR. Furthermore，phosphorylation of USP8 at Ser680 results in its binding of 14-3-3，catalytic inactivation，and reduced EGFR deubiquitination. It appears as though USP8，in conjunction with components of the ESCRT-0 complex，plays an integral role in the early endosomal sorting machinery that functions to protect EGFR from lysosomal degradation.
应用类型
Western blot
免疫原
Synthetic peptide corresponding to an internal sequence of human USP 8 (BV-L43)
