Ubiquitinating enzymes （UBEs） catalyze protein ubiquitination，a reversible process countered by deubiquitinating enzyme （DUB） action. Five DUB subfamilies are recognized，including the USP，UCH，OTU，MJD，and JAMM enzymes. The deubiquitinating enzyme ubiquitin-specific protease 8 （USP8/UBPy） is a cysteine protease belonging to the USP/UBP subfamily. Research studies have shown that USP8 is an essential growth-regulated enzyme indispensible for cell proliferation and survival. Indeed，conditional knock-out of murine USP8 was shown to promote a dramatic loss in expression of receptor tyrosine kinases，including EGFR，ErbB3，and c-Met. In agreement with these findings，USP8 inactivation leads to enhanced ubiquitination of ligand-activated EGFR. Furthermore，phosphorylation of USP8 at Ser680 results in its binding of 14-3-3，catalytic inactivation，and reduced EGFR deubiquitination. It appears as though USP8，in conjunction with components of the ESCRT-0 complex，plays an integral role in the early endosomal sorting machinery that functions to protect EGFR from lysosomal degradation.
应用类型
Western blot: 1-4 ug/ml.
免疫原
Synthetic peptide corresponding to an internal sequence of human USP 8 （BV-L43）
