Protein ubiquitination and Deubiquitination are reversible processes catalyzed by ubiquitinating enzymes （UBEs） and deubiquitinating enzymes （DUBs）. DUBs are categorized into 5 subfamilies: USP，UCH，OTU，MJD，and JAMM. UCHL1，UCHL3，UCHL5/UCH37，and BRCA-1-associated protein-1 （BAP1） belong to the UCH family of DUBs，which all possess a conserved catalytic domain （UCH domain） of about 230 amino acids. Although UCHL1 and UCHL3 are the most closely related UCH family members with about 53% identity，their biochemical properties differ in that UCHL1 binds monoubiquitin and UCHL3 shows dual specificity toward both ubiquitin （Ub） and NEDD8，a Ub-like molecule. UCHL1 （PGP9.5） is a 25 kDa protein； it is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. It comprises >1% of total brain protein but is almost absent from other tissues. It has been implicated both in Parkinson’s disease and in lung cancer.
应用类型
Western blot: Robust detection of 100 ng of recombinant protein was possible when antibody was used at a final concentration of 5 ug/mL
