Jumonji Domain containing 2A (JMJD2A) is a lysine specific demethylase with emerging roles in histone modification or epigenetic remodeling. This JMJD2A polyclonal antibody was raised against an N-terminal recombinant fragment of JMJD2A. This fragment includes amino acids 1-350 including the JMJN and JMJC domains but not the two LAP/PHD zinc finger or Tudor domains of the 1,064 amino acid protein. JMJD2A is detected at 135 kDa by western blotting using a DLD1 cell lysate as a positive control. Also，the expression of JMJD2A varies by tissue，and northern blotting sμggests skeletal muscle as a negative control and lung as a positive control tissue.
应用类型
Western blot
免疫原
Human recombinant JMJD2A aa 1-350
来源宿主
Rabbit
反应性
Human JMJD2A. Expected to cross react with JMJD2C (>90% similarity), JMJD2B (>85% similarity) due to high homology to these JMJD2 paralogs considering the antigen used. Observed detection of purified recombinant human JMJD2D at 50 ng sμggesting only ~10% cross reactivity with recombinant JMJD2D, however bands were detected at 60 kDa from cell samples.
