The sirtuins represent a distinct class of trichostatin A-insensitive lysyl-deacetylases （class III HDACs） and have been shown to catalyze a reaction that couples lysine deacetylation to the formation of nicotinamide and O-acetyl-ADP-ribose from NAD+ and the abstracted acetyl group. SIRT7 is a member of this family of proteins and is present in prokaryotes and eukaryotes. All SIR2-like proteins have a sirtuin core domain，which contains a series of sequence motifs conserved in organisms ranging from bacteria to humans. Bacterial，yeast，and mammalian sirtuins are able to metabolize NAD+ and several act as mono-ADP-ribosyltransferases. The enzymatic function of sirtuins is not yet completely understood but as mentioned above，recent reports of histone-activated SIR2-mediated NAD+ metabolism and NAD+-activated SIR2-mediated histone deacetylation suggest a possible coupled reciprocal activation mechanism involving interactions of SIR2 with NAD+ and the N-ε-acetyl-lysine groups of acetylated histone.
应用类型
Western blot: 1-3 ug/ml. However， the optimal conditions should be determined individually.
