Accumulation of the amyloid-B peptide （AB） in the cerebral cortex is a critical event in the pathogenesis of Alzheimer’s disease. The B-amyloid protein precursor （APP） is cleaved by B-secretase，producing a soluble derivative of the protein and a membrane anchored 99-amino acid carboxy-terminal fragment （C99）. The C99 fragment serves as substrate for g-secretase to generate the 4 kDa amyloid-B peptide，which is deposited in the brains of all suffers of Alzheimer’s disease.
应用类型
Western blot analysis （1-4 ug/ml） and Immunohistochemistry （2-10 ug/ml）. However， the optimal conditions should be determined individually. Murine and rat brain tissues can be used as positive controls.
免疫原
Synthetic peptide from human APP
来源宿主
兔
反应性
经检测验证，可与人、小鼠、大鼠种属来源的APP蛋白发生免疫反应.
保存建议
提供的APP抗体产品为100 ug 无色溶液，其浓度为0.5 mg/ml，溶解于包含有50%甘油， 1% BSA， 0.02%硫柳汞的PBS （pH 7.2）缓冲液中.请将该APP抗体产品置于-20 ℃冷冻保存，建议分装使用以避免反复冻融.
