In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. Alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. Beta and gamma actins coexist in most cell types as components of the cyto-skeleton and as mediators of internal cell motility. Beta actins are highly conserved proteins that are involved in cell motility, structure and integrity. Beta actins are cytoplasmic proteins. Anti-Actin is a loading control antibody and is critical for the correct interpretation of your western blot. Beta-Actin Loading Control Antibody is used to normalize the levels of protein detected by confirming that protein loading is uniform across the gel.
应用类型
ELISA,IF Microscopy,Western Blot,
免疫原
beta-Actin Loading Control Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal region near amino acids 350-375 of Human beta Actin.
来源宿主
Rabbit
反应性
H. sapiens (Human); Lithobates (Leopard frog); Mus musculus (Mouse)
保存建议
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
